Single nucleotide analysis of cytosine methylation by whole-genome shotgun bisulfite sequencing.
作者： Michelle D. Johnson , Michael Mueller , Laurence Game , Timothy J. Aitman
DOI: 10.1002/0471142727.MB2123S99
关键词:
摘要: Unlike other methods to assess methylation, whole-genome shotgun bisulfite sequencing is used generate quantitative genome-wide methylation profiles at single-nucleotide resolution. As described in this unit, method allows for the measurement of each cytosine base by treatment genomic DNA with sodium followed and alignment reads a reference genome. Curr. Protoc. Mol. Biol. 99:21.23.1-21.23.28. © 2012 John Wiley & Sons, Inc. Keywords: DNA methylation; bisulfite sequencing; second-generation sequencing; methylation profiling; read mapping
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