Differential DNA Methylation Correlates with Differential Expression of Angiogenic Factors in Human Heart Failure

摘要: Epigenetic mechanisms such as microRNA and histone modification are crucially responsible for dysregulated gene expression in heart failure. In contrast, the role of DNA methylation, another well-characterized epigenetic mark, is unknown. order to examine whether human cardiomyopathy different etiologies connected by a unifying pattern methylation pattern, we undertook profiling with ischaemic idiopathic end-stage cardiomyopathic left ventricular (LV) explants from patients who had undergone cardiac transplantation compared normal control. We performed preliminary analysis using methylated-DNA immunoprecipitation-chip (MeDIP-chip), validated differential loci bisulfite-(BS) PCR high throughput sequencing, identified 3 angiogenesis-related genetic that were differentially methylated. Using quantitative RT-PCR, found these genes differed significantly between CM hearts control (p<0.01). Moreover, each individual LV tissue, showed predicted correlation corresponding gene. Thus, exists cardiomyopathy. this series heterogenous explants, was at least genes. While other systems, changes specific genomic usually precede genes, our current findings merit further investigation determine play causative progression