Screen and identification of human colorectal carcinoma metastasis- associated genes

摘要: AIM: To primarily screen and identify metastasis-associated genes of colorectal carcinoma to illustrate the molecular mechanisms metastasis. METHODS: Suppression subtractive hybridization was performed between a pair high metastatic cell line SW620 low SW480 human carcinoma, which originated from same parent. Two subtracted cDNA libraries for metastasis accelerating or suppressor had been then constructed. About 200 bacterial PCR fragments were picked out randomly obtain differentially expressed by differential screening (DS) method. After that, expression partial validated Northern blot. Then sequenced. Finally BLAST searching literature review done analyze their characters possible in process carcinoma. RESULTS: Differential about fragments, totally 29 obtained. blot validate 4 fragments. The results consistent with DS findings. sequenced Blast analysis showed that 25 gene There 10 known genes, among 5 lines thus may accelerate metastasis, including heat shock protein10, cytochrome C oxidaseII, mitotic control protein dis3 homolog, skeletal muscle actin alpha1 serum amyloid A. In addition, another have potential suppress included egl nine homolog 2, eukaryotic translation initiation factor 4A, similar oxidase , glutathione S transferase mu 3 mitochondrion DNA. These are mainly related growth differentiation, metabolism synthesis, transcription, apoptosis, signal transduction so on. Except DIS3 SAA, other first reported on relationship studies. 15 unknown screened this study. genome sequences, 6 found be located chromosome 5, candidate accerating 2 Those collected GeneBank dbEST database accession number CD485499-CD485513 respectively. CONCLUSION: method is efficient way expressive library. Changes apoptosis play an important role Chromosome might exist many new locations Liang L, Ding YQ, Li X, Yang YF, Xiao J, Zhang JH, Zhao PR. Screen identification genes. Shijie Huaren Xiaohua Zazhi 2004;12(8):1800-1805