High Performance Cation Exchange Chromatography of Basic Peptides

摘要: Abstract S-Zephyr, a hydrophilic high performance cation exchanger, has been successfully used for the separation of series synthetic basic peptides. Due to absence any hydrophobic character support, linear relationship was found between retention time and number cationic charges. The ability discriminate peptides according their charge, even in case one unit difference, are illustrated total seven peptides, which included mtr-arginine impurity derived from peptide synthesis. Preparative trials overloading conditions were studied each give best 99.2% final purity with dynamic sorption capacity 18 mg per ml sorbent.