Transfer of plasma membrane proteins between cells using reconstituted membrane vesicles as shuttle vehicles.

摘要: Purified membrane glycoproteins from liver or hepatoma tissue culture cells were incorporated in a right-side-out orientation into reconstituted phospholipid vesicles by detergent dialysis method. The phospholipids purified preparations of rat liver. protein:phospholipid ratio the was optimized for efficient transfer vesicle contents to recipient cells, usually mouse L cells. Fluoresceinated albumin lumen used as marker after polyethylene glycol-mediated fusion. redistribution and fate both lipids transferred proteins analysed microscopic biochemical methods. A hepatocyte-specific binding protein galactose- galactosamine-terminated serum set cell plasma successfully fibroblasts these biological function hepatic protein, namely delivery galactose-terminated glycoprotein ligand lysosome degradation, imparted fibroblast transfer. Further, polypeptide carbohydrate moieties degraded at about same relative rates they had original donor fibroblasts. These studies show that technique inserting constituents another can help elucidate route mechanism turnover.