Insertion of biologically active membrane proteins from rat liver into the plasma membrane of mouse fibroblasts.

摘要: Abstract Phospholipids, glucolipids, and total proteins were separated from a plasma membrane fraction of rat liver. Membrane glycoproteins isolated deoxycholate extracts liver membranes hepatoma tissue culture by concanavalin A chromatography. The glycoprotein on hepatocytes that acts as receptor for serum have lost their terminal sialic acid was also purified membranes. Closed vesicles reconstituted mixtures deoxycholate-solubilized phospholipids dialysis isopycnic centrifugation. orientation the in these examined accessibility to trypsin neuraminidase ability be released vesicle different concentrations detergent. Most are embedded right-side-out lipid bilayer. can fused mouse L-cells with polyethylene glycol. extent fusion is function phospholipid:protein ratio vesicles. After fusion, phospholipid component mixes relatively rapidly cell lipids judged immunofluorescence pattern cells containing trinitrophenylated lipids. In contrast, transferred show restricted diffusion again techniques. metabolic turnover after transfer radioisotopic methods. Total very stable L-cells. Some may involved formation an exoskeleton at surface. Hepatoma less terms properties than proteins. However, some into medium large molecular weight material rather being degraded small weight, acid-soluble component. asialoglycoproteins this hepatocyte-specific half-life L-cell least 50 h. Transfer confers upon recipient biological activities specified initiated receptors hepatocytes.