Anin vitrohigh-throughput assay for screening reproductive and toxic effects of anticancer compounds
作者： Vicki Edwards , Kirsten Benkendorff , Fiona Young
DOI: 10.1002/BAB.1199
关键词:
摘要: An in vitro assay was developed that simultaneously tested the effects of anticancer drug candidates on cytotoxicity, hormone synthesis, and gonadotrophin responsiveness using choriocarcinoma JAr cell line. culture conditions were optimized then cells exposed to a marine mollusc extract presence absence hCG. The intra- interassay coefficients variation 1 H thiazolyl blue tetrazolium bromide 11.3% 10.9%, respectively. hCG (1,000 mIU/mL) increased progesterone (P4) synthesis after 24 (P < 0.05). significantly decreased viability, with IC50 affected by incubation time, but not P4 inhibited at low concentrations extract, stimulated highest concentration, complex interactions also found In conclusion, is useful characterize novel drugs basal, gonadotrophin-stimulated vitro, can be used inform subsequent vivo studies. C
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