Rho-Associated Protein Kinase (ROCK) Promotes Proliferation and Migration of PC-3 and DU145 Prostate Cancer Cells by Targeting LIM Kinase 1 (LIMK1) and Matrix Metalloproteinase-2 (MMP-2).

摘要: BACKGROUND In the pathogenesis and progression of prostate cancer, cell proliferation migration results in tumor invasion metastasis that is associated with patient morbidity mortality. Rho-associated protein kinase (ROCK) has previously been shown to be upregulated but its biological role remains poorly understood. This study aimed investigate ROCK PC-3 DU145 cancer cells identify possible targets involved by knockdown ROCK1 ROCK2 RNA expression. MATERIAL AND METHODS An interference (RNAi) assay was performed silence expression human lines. Cells were also treated a specific inhibitor, Y27632. A counting kit-8 (CCK-8) used determine rate cells, assays performed. Western blot polymerase chain reaction measure levels. RESULTS reduced invasion. regulated cells. Protein levels phosphorylated LIM 1 (p-LIMK1) matrix metalloproteinase-2 (MMP-2) siRNA transfected CONCLUSIONS promoted targeting LIMK1 MMP-2.