Effect of the lipid composition of Mycoplasma mycoides subspecies Capri and phosphatidylcholine vesicles upon the action of polyene antibiotics

摘要: Abstract 1. (1) The effects of filipin and amphotericin methyl ester upon the K+ efflux from Mycoplasma mycoides subsp. capri egg lecithin sonicated vesicles were investigated. Both nature sterol composition membranes affected sensitivity to each polyene antibiotic. 2. (2) M. containing ergosterol was much more sensitive than cells cholesterol. Cholesterol-containing about twice as ergosterol-containing cells. These results confirmed with phosphatidylcholine vesicles. 3. (3) At 2 °C independent membrane cholesterol content towards decreased when increased, in contrast at 20 °C. 4. (4) 2°C, sterol-free became very both presence did not increase further. high concentrations ( > 30 mol % ), antibiotic sensitivity, particularly ester, greatly reduced.