Emergence of G9P[8] rotaviruses in children with acute gastroenteritis in Thailand, 2015‐2016

摘要: Human group A rotavirus is a major contagious virus causing gastroenteritis in children. Molecular epidemiological study of infections hospitalized children was performed by multiplex RT-PCR during 2015-2016 Chiang Rai, Thailand. G- and P-genotypes positive samples were further analyzed one-step two-step methods. Among 270 fecal specimens tested, the most prevalent (33.7%), followed norovirus GII (4.1%), adenovirus (3%), astrovirus (1.5%). Infection common patients aged 12-23 months (45.1%) occurred mostly under 3 years age (85.7%). The highest peak hot month, March (64.8%). G9P[8] emerged as predominant (79.1%), G3P[8] (13.2%), G1P[8] (3.3%), mixed G-types (4.4%). Interestingly, Rai G9 strains clustered within distinct lineage VII including recently reported since 2010-2015. G9-VII also contained four to five unique amino acid substitutions VP7 proteins compared with those candidate vaccine strain RVA/Human-tc/IND/116E/1985/G9P[11] prototype RVA/Human-wt/USA/WI61/1983/G9P[8], defining novel variant. G3 closely related "new reassortant variant" an equine-like gene that several countries. This contributes understanding genetic diversity, providing scientific support for future strategies reduce morbidity mortality.