Chemoselective labeling and site-specific mapping of 5-formylcytosine as a cellular nucleic acid modification.
作者： Julia Dietzsch , Doris Feineis , Claudia Höbartner
关键词:
摘要: DNA methylation has a profound impact on the regulation of gene expression in normal cell development, and aberrant been recognized as key factor pathogenesis human diseases such cancer. The discovery modified nucleobases arising from 5-methylcytosine (5mC) through consecutive oxidation to give 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) stimulated intense research efforts regarding biological functions these epigenetic marks. This Review focuses sensitive detection quantitation 5fC RNA by chemoselective labeling, which aims at discriminating between its thymine counterpart 5-formyluracil (5fU), summarizes single-base resolution sequencing methods for locus-specific mapping 5mC oxidized derivatives.
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