Control of the Cdc6 replication licensing factor in metazoa: the role of nuclear export and the CUL4 ubiquitin ligase.

摘要: A central requirement to maintain genome stability is that DNA replication must be tightly controlled so genomic replicated only once in a single cell cycle. The prevention of re-replication achieved by restricting the assembly pre-replicative complexes (pre-RCs) period prior S phase, and ensuring pre-RCs cannot reform during phase. regulation licensing factors Cdt1 Cdc6 phase critical prevent reformation pre-RCs. In yeast, degraded block re-replication. mammals, exported from nucleus; however, variable percentage endogenous remains nuclear throughout perdurance has led number groups question whether export relevant its activity. recent study C. elegans shows fact This work also identifies CUL-4 ubiquitin ligase as master regulator controls regulating both factors.