Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication.

摘要: Prohibitin, a tumor suppressor protein, has been shown to repress E2F-mediated transcription and arrest cell cycle progression. while prohibitin proposed regulate progression by repressing transcriptional targets of E2F1, it is not clear whether other mechanisms are also involved in mediating the growth arrest. Here we demonstrate that can function as potent inhibitor DNA replication interacting with members Minichromosome maintenance complex proteins (MCM2-7). The data presented here indicates physically interact MCM2, MCM5 MCM7 vitro GST binding assays well MCF-7 cells seen immunoprecipitation-western blot experiments. association was dependent, more pronounced 4-8 hours after serum stimulation quiescent cells. Prohibitin associated robustly MCM2 compared MCM7, suggesting mainly interacts regulatory subunits MCM complex. Confirming these results, found co-localize cells, double immunofluorescence Further, strongly inhibited an assay. These results suggest effectively represses components mammalian machinery this might contribute properties prohibitin.