Structure of the lipid-containing bacteriophage PRD1: disruption of wild-type and nonsense mutant phage particles with guanidine hydrochloride.

摘要: The lipid-containing bacteriophage PRD1 was disrupted, and the subviral particles were studied. Guanidine treatment released two phage proteins (P3 P5). These form polyhedral capsid. remaining associated with membrane vesicle. vesicle capable of forming a tubular structure. isolated vesicles aggregated readily. We found that aggregation tube formation specific (P11 P18, respectively) by using protease an analysis nonsense mutant particles. In addition, possibility free might be precursors to empty virions