Gene expression profiling in Paget's disease of bone: upregulation of interferon signaling pathways in pagetic monocytes and lymphocytes.

摘要: We examined the gene expression profile of genes involved in bone metabolism 23 patients with PD compared healthy controls. found a significant overexpression IFN pathway along downregulation tnf-α. Our result suggest that IFN-mediated signaling may play important roles aberrant osteoclastogenesis PD. Introduction: Paget's disease (PD) is characterized by focal regions highly exaggerated remodeling and osteoclastogenesis. Under physiological conditions, circulating monocytes serve as early progenitors osteoclasts peripheral blood lymphocytes produce wide variety factors metabolism. Nevertheless, little known about relation to pathological turnover PD. Materials Methods: In this study, we aimed at investigating pattern using quantitative real-time PCR isolated from mononuclear cells (PBMCs). Fifteen be were studied Eight human including ifn-α (3.48-fold, p < 0.001), ifn-β (2.68-fold, ifn-γ (1.98-fold, = 0.002), p38 β2 mapk (2.47-fold, ifn-γr1 (2.03-fold, 0.01), ifn-γr2 (1.81-fold, 0.02), stat1 (1.57-fold, 0.037), tnf-α (−2.34, 0.001) significantly altered pagetic controls. Results: lymphocytes, changes (2.17-fold, (2.13-fold, 0.005), (1.89-fold, (1.02-fold, 0.04), (1.01-fold, 0.031), stat2 (1.79-fold, (−1.49, Furthermore, IFN-γ protein was elevated sera (18.7 ± 6.69 pg/ml) controls (3.87 6.48 pg/ml, 0.042). Conclusions: conclusion, our data novel pathways mainly related PD.