Hammerhead Ribozyme as a Therapeutic Agent for Hyperlipidemia: Production of Truncated Apolipoprotein B and Hypolipidemic Effects in a Dyslipidemia Murine Model
作者： Munechika Enjoji , Fang Wang , Makoto Nakamuta , Lawrence Chan , Ba-Bie Teng
DOI: 10.1089/104303400750038516
关键词:
摘要: In humans, overproduction of apolipoprotein B (apoB) is positively associated with premature coronary artery diseases. To reduce the levels apoB mRNA, we used adenovirus-mediated vector to target hammerhead ribozyme at GUA6679 ↓ mRNA (designated AvRB15) in liver a dyslipidemic mouse model that deficient editing enzyme and overexpresses human apoB100. this study, delivered ~4 × 1011 virus particles AvRB15 (active ribozyme) or AvRB15-mutant (inactive animals. Using Southern blot analysis, readily detected RB15 DNA as long day 35 after injection. This result was correlated RNA expression by RNase protection assay. reverse ligation-mediated polymerase chain reaction, 3′ cleavage product detected, exact site confirmed sequencing. Importantly, decreased ~80% transduction. There marked decrease plasma...
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