Human recombinant IL-6/B cell stimulatory factor 2 augments murine antigen-specific antibody responses in vitro and in vivo.

作者: F Takatsuki , A Okano , Y Akiyama , T Kishimoto , T Hirano

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摘要: The effects of human rIL-6/B cell stimulatory factor 2 (hrIL-6/BSF-2) from Escherichia coli on murine Ag, SRBC-specific antibody responses were examined in vitro and vivo. HrBSF-2 was effective augmenting the primary anamnestic plaque-forming cells response to SRBC vitro. augmentation apparent when B cell-enriched spleen (B cells) cultured with BSF-2 presence IL-2. On other hand, hrBSF-2 alone strongly enhanced a dose-dependent manner SRBC-immunized mice used. These abolished completely by anti-BSF-2 antibody, but not normal rabbit Ig. Cell depletion experiments indicated that L3T4 (CD4)+ T cells, Lyt-2(CD8)+ adherent (macrophages) have an important role this BSF-2-induced response. In addition, kinetic studies showed acts even added relatively late culture. Finally, it determined whether also could be active modulating shown enhance secondary mice. most effect observed

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