Deglycosylation of flavonoid O-glucosides by human intestinal bacteria Enterococcus sp. MRG-2 and Lactococcus sp. MRG-IF-4

摘要: Daidzin, daidzein 7-O-glucoside, is a major isoflavone in soybean and acts as phytoestrogen. By intestinal bacteria dietary, daidzin hydrolyzed to the aglycone further converted more reduced metabolites, such dihydrodaidzein, tetrahydrodaidzein, equol. Human Enterococcus sp. MRG-2 Lactococcus MRG-IF-4, which convert daidzein, were isolated under anaerobic condition, identified by 16S rRNA gene sequence analysis. Changes of OD600 pH measured during growth, deglycosylation kinetics genistin HPLC. Both also other 7-O-glucosides, glycitin, ononin, sissotrin, their aglycones, glycitein, formononetin, biochanin A, respectively. Apigetrin was deglycosylated apigenin these too. However, rutin, hesperidin, naringin not aglycones. Phylogeny analysis strains found that bacterial species correlated with its metabolic ability flavonoid biotransformation.