Differential expressions of cancer-associated genes and their regulatory miRNAs in colorectal carcinoma.

摘要: Colorectal cancer is one of the frequently seen malignancies in world. To date, several oncogenes and tumor suppressor genes have been identified linked to colorectal pathogenesis. Although recent advances diagnosis therapy are promising, identifying novel genetic contributors still high priority. In present study, expression profile some cancer-related their regulatory miRNA molecules were evaluated by using a high-throughput real-time PCR method. For total 54 patients diagnosed with CRC normal colon tissue samples 42 healthy controls included. analysis, RNA was extracted from FFPE converted cDNA. All analyses assessed Fluidigm Microfluidic Dynamic Array chips for 96 reactions held BioMark™ HD System Real-Time PCR. As result ADAMTS1, FHIT, RUNX1, RUNX3 WWOX shown be significantly altered tissues contrast samples. Moreover, miR-378a-3p, miR-155-5p, miR-193b-3p, miR-96-5p, miR-17-5p, miR-27a-3p, miR-133b, miR-203a, miR-205-5p, miR-34c-5p, miR-130a-3p, miR-301a-3p, miR-132-3p, miR-222-3p, miR-34a-5p, miR-21-5p, miR-29a-3p miR-29b-3p found deregulated CRC. Consequently, results current study strongly suggest involvement miRNAs physiopathology.