Replication timing aberrations and aneuploidy in peripheral blood lymphocytes of breast cancer patients.

摘要: BACKGROUND: Peripheral blood lymphocytes of patients with hematological malignancies or solid tumors, such as renal cell carcinoma prostate cancer, display epigenetic aberrations (loss synchronous replication allelic counterparts) and genetic changes (aneuploidy) characteristic the cancerous phenotype. This study sought to determine whether alterations could differentiate breast cancer from cancer-free subjects. METHODS: The HER2 locus—an oncogene assigned chromosome 17 whose amplification is associated (BCA)—and pericentromeric satellite sequence (CEN17) were used for timing assessments. Aneuploidy was monitored by enumerating copy numbers 17. Replication aneuploidy detected cytogenetically using fluorescence in situ hybridization technology applied phytohemagglutinin-stimulated 20 women BCA 10 control RESULTS: We showed that both CEN17 loci stimulated altered their pattern exhibited asynchronicity. In addition, there an increase aneuploidy. frequency cells displaying asynchronous patients' samples significantly higher ( P −12 −6 ) than corresponding values samples. Similarly, −9 controls. CONCLUSIONS: aberrant differentiated clearly between Thus, monitoring these genes offers potential markers detection cancer.