[24] Methods for molecular cloning in eukaryotic cells

摘要: Publisher Summary This chapter describes the methods for molecular cloning in eukaryotic cells. The discovery and purification of bacterial restriction enzymes have facilitated isolation specific deoxyribonucleic acid (DNA) fragments spanning entire operons. These DNA can be joined vitro to replicons Escherichia coli ( E. )—such as a plasmid or bacteriophage λ genome—and replication amplification these molecules accomplished host been used clone wide variety prokaryotic segments . discusses use papovavirus vectors mammalian Several other potential systems that not yet employed cells are described chapter. Although selected joining vector foreign may vary depending on nature their terminal sequences, one example is illustrated explain way SV40 genome utilized cloning. construction hybrid between argF gene