Viral DNA synthesis in cells infected by temperature-sensitive mutants of simian virus 40.

作者: Janice Yang Chou , Jesus Avila , Robert G. Martin

DOI: 10.1128/JVI.14.1.116-124.1974

关键词:

摘要: Temperature-sensitive mutants of simian virus 40 (SV40) have been classified as those that are blocked prior to viral DNA synthesis at the restrictive temperature, "early" mutants, and harboring a defect later in replication cycle, "late" mutants. Mutants A D complementation groups early, B, C, BC late. Our results confirm earlier reports defective function required for initiation each round synthesis. on other hand, continue temperature after preincubation permissive temperature. The length time be expressed temperature-after which infection proceeds unabated shifting cultures temperature-is 10 20 h. synthesized under these conditions progresses normal fashion through replicative intermediate molecules mature component I II molecules.

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