Myricetin treatment induces apoptosis in canine osteosarcoma cells by inducing DNA fragmentation, disrupting redox homeostasis, and mediating loss of mitochondrial membrane potential

作者: Hahyun Park , Sunwoo Park , Fuller W. Bazer , Whasun Lim , Gwonhwa Song

DOI: 10.1002/JCP.26598

关键词:

摘要: Canine osteosarcoma is an aggressive primary bone tumor that shows metastasis to distal regions and associated with a high mortality rate. However, the pathophysiological mechanisms of canine are not well characterized. In addition, development prognostic factors novel therapeutic agents necessary efficiently treat osteosarcoma. Therefore, we studied effects myricetin, antioxidant found in berries, nuts, teas, wine, vegetables, on apoptosis signal transduction cell lines, D-17 DSN. Results present study demonstrated treatment myricetin decreased proliferation DNA replication, while it increased apoptotic fragmentation DSN cells. generation ROS, lipid peroxidation, depolarization MMP both Myricetin activated phosphorylation AKT, p70S6K, ERK1/2, JNK, p90RSK Moreover, inhibition PI3K MAPK using LY294002, U0126, or SP600125, addition treatment, effectively suppressed compared each inhibitor alone. concluded may be potentially effective less toxic agent prevent control progression

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