作者: Zhao Wenqi , Tan Yanwen , Zhang Yuanbo , Zhou Lei , Qiu Meng
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摘要: The invention belongs to the technical field of biophysical single-molecule observation surface treatment, and particularly discloses a method using thin-layered graphene fluorescence quenching combined with nano-metal array perform observation. comprises following specific steps: processing area; preparing ordered sequence on substrate. According disclosed by invention, properties graphene, such as fluorescent molecules in certain area surface, having good permeability, enabling loss exciting light signals be lowered, are utilized; metal nano-structure can normally molecules, layer longitudinally lengthens distance between so that generate beyond range; concentration joining condition controlled realize