Combined Campylobacter jejuni and Campylobacter coli Rapid Testing and Molecular Epidemiology in Conventional Broiler Flocks.

摘要: Campylobacter spp. are important causes of bacterial zoonosis, most often transmitted by contaminated poultry meat. From an epidemiological and risk assessment perspective, further knowledge should be obtained on prevalence genotype distribution in primary production. Consequently, 15 Austrian broiler flocks were surveyed summer for their thermophilic contamination status. Chicken droppings, dust drinking water samples collected from each flock at three separate sampling periods. Isolates confirmed PCR subtyped. We also compared alternative methods (culture-based enrichment Bolton broth, culture-independent real-time a lateral-flow test) applicability chicken droppings. Twelve found to positive during the entire period. Seven (46.6%) with both, C. jejuni coli, five harboured solely one species. observed majority flock-specific coli genotypes, which dominated respective flock. Flocks within distance <2 km shared same genotypes indicating cross-contamination event via environment or personnel vectors. Multilocus sequence typing (MLST) revealed that isolates assigned globally distributed clonal complexes had strong link human interface (CC ST-446 ST4373). The combination techniques poses advantage over studies based cultures alone, as, case Campylobacter, occurrence high variety might present among suggest applying test under field conditions identify 'high-shedding' farm level. farmers veterinarians could improve hygiene measurements direct sanitation activities, especially thinning Ultimately, applied quantify directly droppings avoid non-interpretable results achieved culture-dependent methods.