Easy-to-use rapid test for direct detection of Campylobacter spp. in chicken feces.

作者: M. WADL , T. PÖLZLER , G. FLEKNA , L. THOMPSON , J. SLAGHUIS

DOI: 10.4315/0362-028X-72.12.2483

关键词:

摘要: Human campylobacteriosis is the leading cause of acute bacterial gastroenteritis in developed countries. One important source infection poultry. Results from Dutch Campylobacter Risk Management and Assessment project indicate that meat broiler flocks shedding $7 log CFU per g feces poses greatest risk transmitting campylobacteriosis. The objective this study was to develop a simple rapid test would identify chicken high numbers Campylobacter. We used lateral flow technology as alternative method, selected culture method according International Organization for Standardization guidelines. To evaluate under field conditions, we sampled either droppings at farms or cecal contents slaughterhouse. PCR confirm presumptive spp. colonies. Under laboratory containing $6.7 CFU/g jejuni $7.1 coli were identif...

参考文章(17)
JOHN M. KRAMER, JENNIFER A. FROST, FREDERICK J. BOLTON, DAVID R. A. WAREING, Campylobacter contamination of raw meat and poultry at retail sale: identification of multiple types and comparison with isolates from human infection. Journal of Food Protection. ,vol. 63, pp. 1654- 1659 ,(2000) , 10.4315/0362-028X-63.12.1654
N. J. STERN, M. C. ROBACH, Enumeration of Campylobacter spp. in Broiler Feces and in Corresponding Processed Carcasses Journal of Food Protection. ,vol. 66, pp. 1557- 1563 ,(2003) , 10.4315/0362-028X-66.9.1557
B Wegmüller, J Lüthy, U Candrian, Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products. Applied and Environmental Microbiology. ,vol. 59, pp. 2161- 2165 ,(1993) , 10.1128/AEM.59.7.2161-2165.1993
S.J Evans, A.R Sayers, A longitudinal study of campylobacter infection of broiler flocks in Great Britain Preventive Veterinary Medicine. ,vol. 46, pp. 209- 223 ,(2000) , 10.1016/S0167-5877(00)00143-4
Akke Vellinga, Frank Van Loock, The dioxin crisis as experiment to determine poultry-related campylobacter enteritis. Emerging Infectious Diseases. ,vol. 8, pp. 19- 22 ,(2002) , 10.3201/EID0801.010129
Hanne Rosenquist, Helle M. Sommer, Niels L. Nielsen, Bjarke B. Christensen, The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter International Journal of Food Microbiology. ,vol. 108, pp. 226- 232 ,(2006) , 10.1016/J.IJFOODMICRO.2005.12.007
Sean F. Altekruse, Norman J. Stern, Patricia I. Fields, David L. Swerdlow, Campylobacter jejuni—An Emerging Foodborne Pathogen Emerging Infectious Diseases. ,vol. 5, pp. 28- 35 ,(1999) , 10.3201/EID0501.990104
Maarten J. Nauta, Arie H. Havelaar, Risk-based standards for Campylobacter in the broiler meat chain Food Control. ,vol. 19, pp. 372- 381 ,(2008) , 10.1016/J.FOODCONT.2007.04.016
D. Acheson, B. M. Allos, Campylobacter jejuni Infections: Update on Emerging Issues and Trends Clinical Infectious Diseases. ,vol. 32, pp. 1201- 1206 ,(2001) , 10.1086/319760
Iain A Gillespie, Sarah J O’Brien, Jennifer A Frost, Goutam K Adak, Peter Horby, Anthony V Swan, Michael J Painter, Keith R Neal, Campylobacter Sentinel Surveillance System Collaborators, None, A Case-Case Comparison of Campylobacter coli and Campylobacter jejuni Infection: A Tool for Generating Hypotheses Emerging Infectious Diseases. ,vol. 8, pp. 937- 942 ,(2002) , 10.3201/EID0809.010817