Resemblance of Carbocycle Formation from Carbohydrates between Archaea and Eucarya/Eubacteria. Biosynthesis of Calditol, the Characteristic Lipid-Content Molecule inSulfolobus acidocaldarius

摘要: We investigated the biosynthesis of unique structure calditol, a part characteristic lipid molecules in major thermophilic archaea, Sulfolobus sp. Deuterium-labeling experiments with deuterated glucose substrates, were carried out. The results clarified that calditol was biosynthesized from C-C bond formation between C-1 and C-5, no loss deuterium at C-6 observed. Two reaction mechanisms possible when these experimental findings, conventional reports considered. Among these, course assumes C-4 oxidation as starting point would be preferable resemblance to myo-inositol biosynthesis. Furthermore, high incorporation suggests activation, such C-1, is not involved ether C-O bonding.