The molecular steps of citrinin biosynthesis in fungi
作者： Yi He , Russell J. Cox
DOI: 10.1039/C5SC04027B
关键词:
摘要: The individual steps of citrinin 1 biosynthesis in Monascus ruber M7 were determined by a combination targeted gene knockout and heterologous expression Aspergillus oryzae. pathway involves the synthesis an unreduced trimethylated pentaketide 10 non-reducing polyketide synthase (nrPKS) known as CitS. Reductive release yields keto-aldehyde 2 first enzyme-free intermediate. nrPKS appears to be assisted as-yet cryptic hydrolysis step catalysed CitA which was previously wrongly annotated oxidase. CitB is non-heme iron oxidase oxidises 12-methyl alcohol. Subsequent are CitC 12-alcohol aldehyde CitD converts 12-aldehyde carboxylic acid. Final reduction C-3 CitE citrinin. rules out alternatives involving intramolecular rearrangements, fully defines molecular for time corrects previous errors literature. activity links fungal tropolone observation aminated shunt products azaphilone biosynthesis. Production coordinated production CitS + CitA–CitE host A. oryzae, each driven constitutive promoter, achieved high yield.
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