Metabolism of platelet-activating factor in human platelets. Transacylase-mediated synthesis of 1-O-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine.
作者： R M Kramer , G M Patton , C R Pritzker , D Deykin
DOI: 10.1016/S0021-9258(18)90696-4
关键词:
摘要: The present study demonstrates that inactivation of exogenous 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (alkylacetyl-GPC; platelet-activating factor) by human platelets is mediated the sequential action two enzymes, 1) a Ca2+-independent acetylhydrolase recovered in cytosolic fraction deacylates alkylacetyl-GPC forming alkyllyso-GPC and 2) CoA-independent, N-ethylmaleimide-sensitive transacylase associated with platelet membranes incorporates long-chain fatty acid into to produce alkylacyl-GPC. Separation phospholipids subsequent resolution individual molecular species high-performance liquid chromatography revealed newly formed alkylacyl-GPC was exclusively alkylarachidonoyl-GPC arachidonoyl group for acylation provided phosphatidylcholine. We conclude previously described (Kramer, R.M., Deykin, D. (1983) J. Biol. Chem. 258, 13806-13811) may play an important role metabolism factor.
elsevier.com
sci-hub.st 参考文章(30)
Charles L. Swendsen, J.Marshall Ellis, Floyd H. Chilton, Joseph T. O'Flaherty, Robert L. Wykle, 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine: a novel source of arachidonic acid in neutrophils stimulated by the calcium ionophore A23187. Biochemical and Biophysical Research Communications. ,vol. 113, pp. 72- 79 ,(1983) , 10.1016/0006-291X(83)90433-3
Peter M. Henson, Activation of Rabbit Platelets by Platelet-Activating Factor Derived from IgE-Sensitized Basophils: CHARACTERISTICS OF THE AGGREGATION AND ITS DISSOCIATION FROM SECRETION Journal of Clinical Investigation. ,vol. 60, pp. 481- 490 ,(1977) , 10.1172/JCI108799
R. L. Wykle, D. A. Bass, C. E. McCall, J. C. Lewis, J. T. O'Flaherty, L. R. DeChatelet, M. Waite, C. H. Miller, 1-O-Alkyl-sn-glyceryl-3-phosphorylcholines: a novel class of neutrophil stimulants. American Journal of Pathology. ,vol. 103, pp. 70- 78 ,(1981)
F Alonso, M G Gil, M Sánchez-Crespo, J M Mato, Activation of 1-alkyl-2-lysoglycero-3-phosphocholine. Acetyl-CoA transferase during phagocytosis in human polymorphonuclear leukocytes. Journal of Biological Chemistry. ,vol. 257, pp. 3376- 3378 ,(1982) , 10.1016/S0021-9258(18)34785-9
R M Kramer, D Deykin, Arachidonoyl transacylase in human platelets. Coenzyme A-independent transfer of arachidonate from phosphatidylcholine to lysoplasmenylethanolamine. Journal of Biological Chemistry. ,vol. 258, pp. 13806- 13811 ,(1983) , 10.1016/S0021-9258(17)43989-5
D H Albert, F Snyder, Biosynthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) from 1-alkyl-2-acyl-sn-glycero-3-phosphocholine by rat alveolar macrophages. Phospholipase A2 and acetyltransferase activities during phagocytosis and ionophore stimulation. Journal of Biological Chemistry. ,vol. 258, pp. 97- 102 ,(1983) , 10.1016/S0021-9258(18)33225-3
Linda M Mcmanus, D. J. Hanahan, R. N Pinckard, K. S. Ferrigni, J. O. Shaw, Activation of human neutrophils with 1-O-hexadecyl/octadecyl-2-acetyl-sn-glycerol-3-phosphorylcholine (platelet activating factor). Journal of Immunology. ,vol. 127, pp. 1250- 1254 ,(1981)
F H Chilton, J T O'Flaherty, J M Ellis, C L Swendsen, R L Wykle, Selective acylation of lyso platelet activating factor by arachidonate in human neutrophils. Journal of Biological Chemistry. ,vol. 258, pp. 7268- 7271 ,(1983) , 10.1016/S0021-9258(18)32170-7
H W Mueller, J T O'Flaherty, R L Wykle, Biosynthesis of platelet activating factor in rabbit polymorphonuclear neutrophils. Journal of Biological Chemistry. ,vol. 258, pp. 6213- 6218 ,(1983) , 10.1016/S0021-9258(18)32394-9
R M Kramer, J A Jakubowski, R Vaillancourt, D Deykin, Effect of membrane cholesterol on phospholipid metabolism in thrombin-stimulated platelets. Enhanced activation of platelet phospholipase(s) for liberation of arachidonic acid. Journal of Biological Chemistry. ,vol. 257, pp. 6844- 6849 ,(1982) , 10.1016/S0021-9258(18)34506-X