Purification of α‐toxin from Clostridium perfringens type A in PEG‐phosphate aqueous two‐phase systems: a factorial study
作者： Maria TH Cavalcanti , Tatiana S Porto , Benicio B Neto , José L Lima‐Filho , Ana LF Porto
DOI: 10.1002/JCTB.1798
关键词:
摘要: BACKGROUND: Purification of α-toxin produced by Clostridium perfringens type A in aqueous two-phase systems (ATPS) was studied with a full two-level factorial design on two factors (concentrations 8000gmol −1 PEG and phosphate salt at pH 8.0), to estimate the influence these purification results. RESULTS: The partition coefficient (K), factor (PF) activity yield (Y) were strongly influenced concentrations. Raising levels increased responses. highest (5.7) obtained concentrations 17.5% 15%, respectively.
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