Subchronic toxicity of aniline hydrochloride in rats

摘要: Hematological, biochemical and histopathological responses of subchronic exposure to aniline hydrochloride (AH) have been investigated in rats. Male Sprague-Dawley rats were given 600 ppm AH drinking water while the control received tap only. Five from each group sacrificed at 30, 60, 90 days treatment. Organ-to-body weight ratio for spleen AH-treated was 56, 61, 53% higher than controls 90, respectively. Liver showed a biphasic pattern this ratio, decrease 30 then an increase 60 days. Among other organs, testes significant Hematological analysis 65% WBC counts rats, whereas, no changes recorded later time points. Erythrocyte very decreases all points, hemoglobin hematocrit decreased Mean corpuscular volume mean increased Methemoglobin content increases 89, 59 45% serum immunoglobulins, IgA groups 24 51 % Analysis splenic lymphocyte subpopulation T-helper (CD4+/CD8−) sub-set subpopulations not affected. Aniline hydroxylase activity liver microsomes significantly At times, demonstrated striking including marked red pulp expansion due sinusoidal cells, fibroblasts, markedly light brown pigment heme origin. Focal pericapsular fibrosis found with evidence neoplasia. These histological greatly accentuated progression exposure. Our data, apart indicating toxicity hemopoietic system, show good interrelationship between damage erythrocytes lesions associated