Cross-Protective Immunity to Leishmania amazonensis is Mediated by CD4+ and CD8+ Epitopes of Leishmania donovani Nucleoside Hydrolase Terminal Domains.

摘要: The Nucleoside hydrolase of Leishmania donovani (NH36) is a phylogenetic marker high homology among parasites. In mice and dog vaccination NH36 induces CD4+ T cell-driven protective response against chagasi infection directed its C-terminal domain (F3). N-terminal vaccines also decreased the footpad lesion caused by amazonensis. We studied basis crossed immune using recombinant generated peptides covering whole sequence saponin for prophylaxis L. F1 (amino acids 1-103) F3 peptide 199-314) enhanced IgG IgG2a anti-NH36 antibodies to similar levels. vaccine induced strongest DTH response, highest proportions NH36-specific CD8+ cells after challenge expression IFN-γ TNF-α. vaccine, on other hand, weaker but significant mild enhancement TNF-α in vivo depletion with anti-CD4 or CD8 monoclonal disclosed that cross-protection amazonensis was mediated cell (75% reduction size lesion) followed (57% lesions). Both were capable inducing long-term cross-immunity. amino acid showed 93% identity NH A34480 which presence completely conserved predicted epitopes domain, differing single acid, domains. identification domains as targets model amazonesis represents rationale development bivalent leishmaniasis.