Polychromatic Flow Cytometry

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DOI: 10.1128/9781555818722.CH17

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摘要: For nearly half a century, flow cytometry has been tool used by biologists to study features of individual cells in rapid and unbiased manner. The measurement cellular relied upon intrinsic properties the which can be examined laser-light scattering or addition extrinsic fluorescent probes, such as dyes fluorochrome-conjugated antibodies that make quantifiable. early cytometers on only one two lasers, mercury arc bulb, for excitation light, generally measured parameter. In late 1970s, Stohr (1) Dean Pinkel (2) described dual-laser cytometry, permitted simultaneous use spectrally distinct fluorochromes, signaling genesis polychromatic cytometry. At roughly same time, Loken colleagues proposed method overcome difficulties caused spectral overlap fluorochromes excited off single laser—a we now refer “compensation” (3). These developments laid foundation future high-polychromatic bases both multi-laser excitations well exciting multiple each laser had described.

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