[18] Peptides and proteins: Overview and strategy

摘要: Publisher Summary This chapter gives an overview and strategy for peptides proteins. There are many problems in peptide protein structure that can be simply solved by determining molecular weights to better than one mass unit. One of the first applications fast atom bombardment- spectrometer (FAB-MS) was verification and, if necessary, correction DNA sequence a gene coding corresponding protein. It merely requires translating open reading frame into calculating tryptic expected from locations lysine arginine. Matching these values with actual weights, determined FAB-MS, components digest reveals whether or not is correct. Those do match indicate either error posttranslational modification product. Sequence errors usually misidentifications base leads codon representing different amino acid, deletion insertion causing shift.