Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin Paracentrotus lividus.

摘要: Echinoderms, such as the rock-boring sea urchin Paracentrotus lividus, attach temporarily to surfaces during locomotion using their tube feet. They can firmly any substrate and release from it within seconds through secretion of unknown molecules. The composition adhesive, well releasing secretion, remains largely unknown. This study re-analyzed a differential proteome dataset Lebesgue et al. by mapping mass spectrometry-derived peptides P. lividus de novo transcriptome generated in this study. resulted drastic increase mapped proteins comparison previous publication. data were subsequently combined with RNAseq approach identify potential adhesion candidate genes. A gene expression analysis 59 transcripts whole mount situ hybridization led identification 16 potentially involved bioadhesion. In future these could be useful for production synthetic reversible adhesives industrial medical purposes.