The frequency and specificity of Human Neutrophil Antigen Antibodies in a blood donor population

摘要: BACKGROUND: Transfusion-related acute lung injury (TRALI) has been associated with both human leukocyte antigen (HLA) and neutrophil (HNA) antibodies. HNA antibody frequency, specificity, demographic associations have not well defined in the blood donor population. STUDY DESIGN AND METHODS: A subset of 1171 donors (388 nontransfused males, 390 HLA antibody–negative females three or more pregnancies, 393 antibody–positive pregnancies) from a larger Leukocyte Antibody Prevalence Study was tested for immunoglobulin (Ig)G IgM using granulocyte immunofluorescence flow cytometry assay. Additional testing on selected samples included monoclonal immobilization antigen–flow genotyping. RESULTS: Eight were positive (prevalence, 0.7%; 95% confidence interval [CI], 0.3%-1.3%]). Three antibodies (one IgG two IgM) found males 0.8%; CI, 0.2%-2.2%); all panreactive nonspecific. One previously pregnant female had an HNA-1a specificity 0.3%; 0.01%-1.4%). Four demonstrated antibodies, one 1%; 0.3%-2.6%). Two these specific, HNA-4a nonspecific. CONCLUSIONS: occur low frequency population are present male donors. Despite implementation TRALI reduction strategies, still products. Although our data do create case urgent testing, future new developments high-throughput screening, including HNA-3a, may warrant reconsideration.