Degradation of microbial polyester poly(3-hydroxybutyrate) in environmental samples and in culture.

摘要: Poly(3-hydroxybutyrate) [P(3HB)] test-pieces prepared from the polymer produced by Azotobacter chroococcum were degraded in natural environments like soil, water, compost and sewage sludge incubated under laboratory conditions. Degradation terms of % weight loss was maximum (45%) after 200 days incubation at 30°C. The P(3HB)-degrading bacterial cultures (36) isolated showed different degrees degradation overlayer method. extent P(3HB) increases up to 12 30°C for majority cultures. For most efficient optimum concentration 0.3% (w/v). Supplementation soluble carbon sources glucose, fructose arabinose reduced while it almost unaffected with lactose. Though significantly, they comparatively less utilizing copolymer 3-hydroxybutyrate 3-hydroxyvalerate [P(3HB-co-3HV)].