[28] A method for anaerobic column chromatography

摘要: Publisher Summary An anaerobic system consists of a column and an elution buffer vessel, both modified to facilitate assembly operation. During operation the volume eluted fractions removed from is balanced by equivalent input oxygen-free gas. The assembled individual units which air flushed with O 2 -free These could be opened subsequently for making additions or connections among if sufficiently large outflow gas maintained create atmosphere at opening. assembled, operated, low levels contaminating oxygen attention given small details such as keeping slight positive pressure in flushing capillary tubing before terminal connection. Plexiglas eluent vessel two identical compartments are used separately step elutions together gradient elutions. With gradients, connection above liquid needed maintain equal compartments.