A membrane-activatable near-infrared fluorescent probe with ultra-photostability for mitochondrial membrane potentials

摘要: Mitochondrial membrane potential (MMP) is a frequently used indicator for mitochondrial function. Herein, we report photostable near-infrared (NIR) fluorescent dye monitoring MMP. This new probe, named NIMAP, non-fluorescent in aqueous solution and can be activated by cell membranes, providing high fluorescence contrast low background fluorescence. NIMAP has been validated MMP living mammalian cells mice. Due to the large response, background, photostability, excellent tissue penetration resulting from red-shifted excitation emission "optical window" above 600 nm, broad applications of this probe are expected.