Astrocytes Increase ATP Exocytosis Mediated Calcium Signaling in Response to Microgroove Structures

摘要: Following central nervous system (CNS) injury, activated astrocytes form glial scars, which inhibit axonal regeneration, leading to long-term functional deficits. Engineered nanoscale scaffolds guide cell growth and enhance regeneration within models of spinal cord injury. However, the effects micro-/nanosize on astrocyte function are not well characterized. In this study, a high throughput (HTP) microscale platform was developed study behavior micropatterned surfaces containing 1 μm spacing grooves with depth 250 or 500 nm. Significant changes in nuclear elongation alignment patterned were observed, compared flat surfaces. The cytoskeleton components (particularly actin filaments focal adhesions) nucleus-centrosome axis aligned along grooved direction as well. More interestingly, showed enhanced mitochondrial activity lysosomes localized at lamellipodia cells, accompanied by adenosine triphosphate (ATP) release calcium activities. These data indicate that lysosome-mediated ATP exocytosis signaling may play an important role astrocytic responses substrate topology. new findings have furthered our understanding biomechanical regulation cell-substrate interactions, benefit optimization scaffold design for CNS healing.