Ultraviolet microscopy of purine compounds in the yeast vacuole.

作者: G. Svihla , J. L. Dainko , F. Schlenk

DOI: 10.1128/JB.85.2.399-409.1963

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摘要: Svihla, G. (Argonne National Laboratory, Argonne, Ill.), J. L. Dainko, and F. Schlenk. Ultraviolet microscopy of purine compounds in the yeast vacuole. Bacteriol. 85:399–409. 1962.—Yeast cells (Candida utilis Saccharomyces cerevisiae) suspended nitrogen-free medium were exposed to various ultraviolet-absorbing biological compounds, particularly nucleic acid constituents. photomicrography was used locate these substances cells. Purines taken up readily concentrated vacuoles C. but not S. cerevisiae. Crystallization occurred, as observed earlier by other techniques. Neither organism assimilated pyrimidine bases, or nucleosides, at a detectable rate. From selective uptake release some derivatives, it can be concluded that properties vacuolar membrane cytoplasmic differ several respects. Images

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