E1A deregulates the centrosome cycle in a Ran GTPase - dependent manner
作者： Patrizia Lavia , Marco G. Paggi , Alfonso Baldi , Anna Severino , Rosamaria Mangiacasale
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摘要: By means of the yeast two-hybrid system, we have discovered a novel physical interaction between adenovirus E1A oncoprotein and Ran, small GTPase which regulates nucleocytoplasmic transport, cell cycle progression, mitotic spindle organization. Expression elicits induction S phase centrosome amplification in variety rodent lines. The supernumerary centrosomes requires functional RCC1, nucleotide exchange factor for Ran and, hence, network. portion responsible with is extreme NH2-terminal region (amino acids 1–36), also required amplification. In an vitro assay recombinant proteins, wild-type interferes on whereas mutant, deleted from region, does not. addition, detected HPV-16 E7 SV40 large T antigen, two oncoproteins functionally related to E1A. These findings suggest common pathway these eliciting virus-induced genomic instability.
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