Gene Expression in Individual Bovine Somatic Cell Cloned Embryos at the 8-cell and Blastocyst Stages of Preimplantation Development
作者： Dasari AMARNATH , Xiangping LI , Yoko KATO , Yukio TSUNODA
DOI: 10.1262/JRD.19096
关键词:
摘要: Aberrant gene expression in somatic cell nuclear-transferred (NT) embryos due to abnormal epigenetic modifications of the donor nucleus likely accounts for much observed diminished viability and developmental abnormalities. We compared 13 developmentally important genes individual 8-cell blastocyst stage NT produced from adults female cumulus cells adult male skin fibroblast with low high incidences neonatal abnormalities [1, 2]. In vitro-fertilized (IVF) were used as control embryos. Among tested, relative abundance Glut-1, IGF-1R, E-cad, Cx43 transcripts varied significantly between two types at stage. The manganese super oxide dismutase (MnSOD) Stat3 was higher IVF both At stage, there a significant difference only one gene, Bcl-2, Although level Glut-1 did not vary blastocysts, its blastocysts lower than that blastocysts. MnSOD tended be profile any single however, highly variable among independent embryo morphology. present study demonstrated profiles examined Day 9 different are largely indistinguishable.
sci-hub.se 参考文章(59)
A. Gutiérrez-ad´n, D. Rizos, T. Fair, P.N. Moreira, B. Pintado, J. de la Fuente, M.P. Boland, P. Lonergan, Effect of speed of development on mRNA expression pattern in early bovine embryos cultured in vivo or in vitro. Molecular Reproduction and Development. ,vol. 68, pp. 441- 448 ,(2004) , 10.1002/MRD.20113
K. Takeda, K. Noguchi, W. Shi, T. Tanaka, M. Matsumoto, N. Yoshida, T. Kishimoto, S. Akira, Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 3801- 3804 ,(1997) , 10.1073/PNAS.94.8.3801
Yin XiJun Yin XiJun, T Tani, I Yonemura, M Kawakami, K Miyamoto, R Hasegawa, Y Kato, Y Tsunoda, Production of Cloned Pigs from Adult Somatic Cells by Chemically Assisted Removal of Maternal Chromosomes Biology of Reproduction. ,vol. 67, pp. 442- 446 ,(2002) , 10.1095/BIOLREPROD67.2.442
Dimitrios Rizos, Fabian Ward, Pat Duffy, Maurice P. Boland, Patrick Lonergan, Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: Implications for blastocyst yield and blastocyst quality Molecular Reproduction and Development. ,vol. 61, pp. 234- 248 ,(2002) , 10.1002/MRD.1153
Dasari AMARNATH, Yoko KATO, Yukio TSUNODA, Effect of the timing of first cleavage on in vitro developmental potential of nuclear-transferred bovine oocytes receiving cumulus and fibroblast cells. Journal of Reproduction and Development. ,vol. 53, pp. 491- 497 ,(2007) , 10.1262/JRD.18112
D. Viuff, B. Avery, T. Greve, W.A. King, P. Hyttel, Transcriptional activity in in vitro produced bovine two- and four-cell embryos Molecular Reproduction and Development. ,vol. 43, pp. 171- 179 ,(1996) , 10.1002/(SICI)1098-2795(199602)43:2<171::AID-MRD6>3.0.CO;2-O
Fátima Santos, Valeri Zakhartchenko, Miodrag Stojkovic, Antoine Peters, Thomas Jenuwein, Eckhard Wolf, Wolf Reik, Wendy Dean, Epigenetic Marking Correlates with Developmental Potential in Cloned Bovine Preimplantation Embryos Current Biology. ,vol. 13, pp. 1116- 1121 ,(2003) , 10.1016/S0960-9822(03)00419-6
H. Takano, C. Kozai, S. Shimizu, Y. Kato, Y. Tsunoda, Cloning of bovine embryos by multiple nuclear transfer Theriogenology. ,vol. 47, pp. 1365- 1373 ,(1997) , 10.1016/S0093-691X(97)00128-3
Christine Wrenzycki, Heiner Niemann, Epigenetic reprogramming in early embryonic development: effects of in-vitro production and somatic nuclear transfer Reproductive Biomedicine Online. ,vol. 7, pp. 649- 656 ,(2003) , 10.1016/S1472-6483(10)62087-1
Yoko Kato, Tetsuya Tani, Yusuke Sotomaru, Kazuo Kurokawa, Jun-ya Kato, Hiroshi Doguchi, Hiroshi Yasue, Yukio Tsunoda, Eight Calves Cloned from Somatic Cells of a Single Adult Science. ,vol. 282, pp. 2095- 2098 ,(1998) , 10.1126/SCIENCE.282.5396.2095