The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors.

摘要: prp6 and prp9 thermosensitive (ts) mutants are affected in pre-mRNA splicing transport from the nucleus to cytoplasm. PRP6 PRP9 wild-type alleles have been sequenced. DNA sequence analysis reveals homologies 5' 3' non-coding regions, suggesting a common regulation of gene expression. genes encode 899 amino acid 530 protein, respectively. The protein has repeated motifs that evoke helix-loop-helix structures. Both proteins cysteine/histidine loosely related those found zinc finger proteins. substitution some, but not all, these residues by directed mutagenesis critical effect on function. Homology searches reveal two other known be involved nuclear pathway--the yeast PRP11 human U1C proteins--contain similar sequences. five four display similarities addition cysteine histidine residues, indicating they participate biological structures or functions process. In addition, exhibit leucine repeat which may implicated interactions. ts mutations mapped