Yeast Splicing Factors and Genetic Strategies for Their Analysis
作者： Jean D. Beggs
DOI: 10.1007/978-3-662-22325-3_5
关键词: RNA splicing 、 Yeast 、 Nucleotide 、 Genetics 、 Intron 、 Biology 、 Gene 、 Conserved sequence 、 Saccharomyces cerevisiae 、 Small Nuclear Ribonucleoprotein Particle
摘要: Relatively few (2–5%) of the genes in budding yeast Saccharomyces cerevisiae contain introns. Those that do usually only one and this is located close to 5’ end transcript. The MATal RPL8A are exceptions, having two Nevertheless splicing an essential process yeast, as introns occur many genes, including more than 50% ribosomal protein genes. Yeast differ from those higher eukaryotes, not exceeding a hundred nucleotides length, containing highly conserved sequence elements at branchpoint (for review see ref. 1). Despite these differences there considerable evidence mechanism pre-mRNA conserved.
springer.com
sci-hub.st 参考文章(102)
Christine Guthrie, Genetic Analysis of Yeast snRNAs Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles. pp. 196- 211 ,(1988) , 10.1007/978-3-642-73020-7_8
B. Schwer, C. Guthrie, A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis. The EMBO Journal. ,vol. 11, pp. 5033- 5039 ,(1992) , 10.1002/J.1460-2075.1992.TB05610.X
A. Pause, N. Sonenberg, Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF‐4A. The EMBO Journal. ,vol. 11, pp. 2643- 2654 ,(1992) , 10.1002/J.1460-2075.1992.TB05330.X
S.H. Kim, J. Smith, A. Claude, R.J. Lin, The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase. The EMBO Journal. ,vol. 11, pp. 2319- 2326 ,(1992) , 10.1002/J.1460-2075.1992.TB05291.X
M. Plumpton, M. McGarvey, J.D. Beggs, A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes. The EMBO Journal. ,vol. 13, pp. 879- 887 ,(1994) , 10.1002/J.1460-2075.1994.TB06331.X
S. Teigelkamp, M. McGarvey, M. Plumpton, J.D. Beggs, The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA. The EMBO Journal. ,vol. 13, pp. 888- 897 ,(1994) , 10.1002/J.1460-2075.1994.TB06332.X
J. Potashkin, D. Frendewey, A mutation in a single gene of Schizosaccharomyces pombe affects the expression of several snRNAs and causes defects in RNA processing. The EMBO Journal. ,vol. 9, pp. 525- 534 ,(1990) , 10.1002/J.1460-2075.1990.TB08139.X
V. Smith, B.G. Barrell, Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of an RNA-binding domain between humans and yeast. The EMBO Journal. ,vol. 10, pp. 2627- 2634 ,(1991) , 10.1002/J.1460-2075.1991.TB07805.X
J.D. Brown, J.D. Beggs, Roles of PRP8 protein in the assembly of splicing complexes. The EMBO Journal. ,vol. 11, pp. 3721- 3729 ,(1992) , 10.1002/J.1460-2075.1992.TB05457.X
P. Legrain, A. Choulika, The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors. The EMBO Journal. ,vol. 9, pp. 2775- 2781 ,(1990) , 10.1002/J.1460-2075.1990.TB07465.X