Magnetically induced electrostimulation of human osteoblasts results in enhanced cell viability and osteogenic differentiation

摘要: The application of electromagnetic fields to support the bone-healing processes is a therapeutic approach for patients with musculoskeletal disorders. ASNIS-III s-series screw bone stimulation system providing stimulation; however, its influence on human osteoblasts (hOBs) has not been extensively investigated. Therefore, in present study, impact this viability and differentiation hOBs was examined. We used s terms specific experimental test set-up. (EMF, 3 mT, 20 Hz) combined an additional alternating electric field (EMF + EF) (3 Hz, 700 mV). primary conducted times per day 45 min over period 72 h. Unstimulated cells served as controls. Subsequently, viability, gene expression markers pro-collagen type 1 synthesis stimulated corresponding controls were both EMF EF using revealed positive cell moderately increased compared unstimulated Stimulation resulted slightly enhanced collagen osteocalcin; significant increase alkaline phosphatase (1.4-fold) osteocalcin (1.6-fold) levels, notable levels runt-related transcription factor 2 (RUNX-2; 1.54-fold). Our findings demonstrate that regards osteoblastic markers.