cDNA cloning and molecular modeling of procerain B, a novel cysteine endopeptidase isolated from Calotropis procera.
作者： Abhay Narayan Singh , Prity Yadav , Vikash Kumar Dubey
DOI: 10.1371/JOURNAL.PONE.0059806
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摘要: Procerain B, a novel cysteine protease (endopeptidase) isolated from Calotropis procera belongs to Asclepiadaceae family. Purification of the enzyme, biochemical characterization and potential applications are already published by our group. Here, we report cDNA cloning, complete amino acid sequencing molecular modeling procerain B. The derived sequence showed high homology with other papain like plant proteases peptidase C1A superfamily. three dimensional structure active B was modeled using X-ray crystal actinidin (PDB ID: 3P5U), fruits Actinidia arguta. structural aspect enzyme is also discussed.
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