Activation of JNK by TPA promotes apoptosis via PKC pathway in gastric cancer cells

摘要: AIM: JNK cascade plays an important role in cell proliferation, differentiation and apoptosis. However, the exact function of for apoptosis induction remains largely unknown. In this study, activation stimulated by TPA process its signaling transduction pathway gastric cancer cells were investigated determined. METHODS: Expressions mRNA protein detected Northern blot Western blot. Transcription activity was measured transient transfection CAT assay. Apoptotic displayed through staining nucleus with DAPI observed under fluorescence microscope. The apoptotic index determined counting 1000 randomly. RESULTS: rapidly TPA, reached highest peak within 3 hr, then decreased a time-dependent manner, but expression level induced always keeping higher than that untreated cells. Similar pattern seen c-jun TPA. significantly activated transcriptional activator protein-1 TPA-dosedependent manner. Furthermore, mediated PKC pathway. Treatment specific inhibitor, Wortmannin, led to repression even presence More importantly, all these effects associated inducted obviously became smaller rounded, their nuclei condensation fragmentation brightly stained chromatin. suppression resulted decrease dramatically from 32.56 % 8.71 %.