In vitro antitrypanosomal and antiplasmodial activities of crude extracts and essential oils of Ocimum gratissimum Linn from Benin and influence of vegetative stage.

摘要: ETHNOPHARMACOLOGICAL RELEVANCE: Different parts of Ocimum gratissimum Linn are largely used in folk medicine for the treatment many diseases, some which related to parasitical infections as fevers and headaches. In order validate their use clarify plant part possesses best antiparasitic properties, we decided evaluate vitro antiplasmodial antitrypanosomal activities essential oils crude extracts from leaves, stems seeds well cytotoxicity. MATERIALS AND METHODS: The ethanol leaves Benin, were obtained pre full flowering stages. Seeds only stage, also extracted. isolated by hydrodistillation analyzed GC/MS GC/FID. Extracts tested against Trypanosoma brucei Plasmodium falciparum. Cytotoxicity was evaluated Chinese Hamster Ovary (CHO) cells human non cancer fibroblast cell line (WI38) through MTT assay selectivity toxicity assessed Artemia salina Leach. RESULTS: non-volatile more active on than falciparum (3D7). This activity varies according vegetative stage (pre flowering) (seeds, leaves) growth inhibition observed with (IC50=1.66 ± 0.48 μg/mL) (IC50=1.29 0.42 good (SI>10). chemical composition oil aerial (47 compounds), characterized presence main constituents p-cymene, thymol, γ-terpinene, β-myrcene α-thujene, depends stage. contained minor compounds such myrcene (IC50=2.24 0.27μg/mL), citronellal (IC50=2.76 1.55μg/mL), limonene (IC50=4.24 2.27μg/mL), activities. These not toxic Leach had a low cytotoxicity except showed CHO WI38 cells. CONCLUSIONS: Our study shows that can be source agents. is first report about relation between extracted, plant, Benin.