On the factors controlling the reversibility of DNA denaturation.

摘要: The experiments described in this paper distinguish two processes which lead to a reversal of DNA denaturation and the re-formation long-stacked arrays nucleotide pairs. Type I reversibility is very rapid intramolecular process occurs preparations from viral, bacterial animal sources, solvents varying greatly ionic strength. II (“renaturation”, Marmur & Lane, 1960 ; Doty, Marmur, Eigner Schildkraut, 1961 ) has properties an intermolecular process, much slower, only relatively homogeneous at moderately high Data on dependence type upon size composition are consistent with proposal that controlled by GC-rich pair sequences act as “nuclei” for re-establishment long-range order. Under conditions these experiments, such nuclei control re-stacking extremely long sections polynucleotide chain. Consequently, strongly affected molecular weight changes range 0·5 10 × 6 . may, however, be dissociated heating. loss accordingly associated final stages dissociation double helix. However, if complementary chains linked covalently, permanent. (Such material called “reversible” DNA.) interpretation stability secondary structure, especially those involving transformation assays, discussed light results.